Journal: bioRxiv

Article Title: Clinical-grade cryopreservation unlocks transplant-ready human pancreatic and stem cell–derived islets for diabetes therapy

doi: 10.64898/2026.04.25.720819

Figure Lengend Snippet: ( A, B ) Bulk RNA-seq volcano plots comparing fresh versus VR native islets ( A ) and SC-islets ( B ). ( C ) Heatmap of differentially expressed genes in VR versus control SC-islets. ( D, E ) Pathway analyses highlighting enrichment of apoptosis, cytokine signaling, and stress responses in SC-islets (PANTHER categories; box-and-whisker plots). ( F ) Confocal images of Annexin V staining in VR islets and SC-islets, indicating apoptosis. Scale bar = 75 µm. ( G ) qPCR analysis of apoptosis-related gene expression in VR-treated SC-islets (mean log₂ fold change [log₂FC], n = 3–4 per group; Student’s t -test, * = p < 0.05). Abbreviations: FDR, false discovery rate; HSR, heat shock response; ISR, integrated stress response; OSR, oxidative stress response; SC, stem cell; UPR, unfolded protein response; VR, vitrified and rewarmed.

Article Snippet: Then, 5× Annexin V binding buffer (Cat. #99902, Biotium) was diluted in deionized water to prepare 1× binding buffer.

Techniques: RNA Sequencing, Control, Whisker Assay, Staining, Gene Expression

Journal: Journal of Virology

Article Title: Foot-and-Mouth Disease Virus Exhibits an Altered Tropism in the Presence of Specific Immunoglobulins, Enabling Productive Infection and Killing of Dendritic Cells ▿

doi: 10.1128/JVI.02180-10

Figure Lengend Snippet: FMDV infection of moDC is closely associated with cell death and reduced ability to stimulate FMDV-specific T cell proliferation. (A) MoDC were exposed to medium, O1K-Cad2, or O1K-Cad2 IC at an MOI of 10 for 6 h. Phase-contrast microscopy captured the high levels of dying cells, including some exhibiting classical apoptotic morphology (high power; right). (B) MoDC were exposed to medium, O1K-Cad2, or O1K-Cad2 IC at an MOI of 10 for 1 to 8 h. Samples of each culture were labeled with antibodies against FMDV NS protein 3A or with annexin V conjugated to APC, and PI. Death was defined as staining with annexin V, PI, or both. The data presented are relative to medium-only values; R = 0.929 (Pearson's correlation coefficient) for the linear relationship between the proportion of cells positive for FMDV NS proteins and the proportion dying. (C) Purified CD4+ T cells from an FMDV-vaccinated animal were added to autologous moDC at a ratio of 1 DC to 100 T cells, with either medium, a sample of the antigen used for vaccination, purified O1K-Cad2, or purified O1K-Cad2 in IC at an MOI of 5. After 5 days, [3H]thymidine was added to each well and incubated overnight before harvesting and liquid scintillation counting of the cells for incorporated radioactivity. Pokeweed mitogen (PWM) was used as a positive control, and in all cases, counts exceeded 350,000 cpm (not shown). A representative data set is presented as cpm ± SD. *, P = 0.02; Student's t test. All 3 replicates using cells from 2 different animals were significant at this level or above.

Article Snippet: To detect dying cells in moDC cultures, 5 × 10 5 cells were pelleted by centrifugation and resuspended in annexin V binding buffer (MBL, Nagoya, Japan).

Techniques: Infection, Microscopy, Labeling, Staining, Purification, Incubation, Radioactivity, Positive Control

Journal: Scientific Reports

Article Title: Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains

doi: 10.1038/s41598-017-06024-2

Figure Lengend Snippet: S. aureus -induced platelet cell death. ( a ) MFI of platelet-bound DIOC6(3) (b) Annexin V binding (c) PMP concentration or (d) fold increase in active caspase-3 expression were analyzed by flow cytometry after 30 min of exposure to Tyrode’s buffer (NS), TRAP, staphylococcal strains [clinical strains (SaB), S. condimenti (Sc) or S. aureus ATCC 43866 (ATCC43866)] or the A23187 apoptosis inducer as a positive control. The data (mean ± SEM; n = 8 experiments) are expressed as the AU ( a ), % expression ( b ) or fold increase compared with NS ( d ). The PMP concentration (mean ± SEM; n = 6–27 experiments) ( c) is expressed as microparticles/µl. *p < 0.05, **p < 0.01, ****p < 0.0001 (one-way ANOVA with repeated measures and the Bonferroni post hoc test; stimulated vs . unstimulated, NS).

Article Snippet: In brief, after stimulation, platelets were resuspended in Annexin Binding Buffer (MabTag, Oldenburg, Germany) for immunostaining.

Techniques: Binding Assay, Concentration Assay, Expressing, Flow Cytometry, Positive Control

Journal: Scientific Reports

Article Title: Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains

doi: 10.1038/s41598-017-06024-2

Figure Lengend Snippet: Effect of ASA and fluvastatin on S. aureus -induced platelet cell death. ( a ) The MFI of platelet-bound DIOC6(3) ( b ) Annexin V binding ( c ) PMP concentration or ( d ) fold increase in active caspase-3 expression were analyzed by flow cytometry after 30 min of exposure to Tyrode’s buffer (as a negative control, NS), TRAP, staphylococcal strains [clinical strains (SaB), S. condimenti (Sc) or S. aureus ATCC 43866 (ATCC43866)] or the A23187 apoptosis inducer ( as a positive control), with ASA, fluvastatin or water (as a control vehicle). ( a ) The data (mean ± SEM; n = 8) are expressed as AU. (b) The data (mean ± SEM; n = 8) are expressed as the % expression ( c ) The data (mean ± SEM; n = 9–27 experiments) are expressed as microparticles/µl. The data (mean ± SEM; n = 6–15 experiments) are expressed as the fold increase in active caspase-3 expression. **p < 0.01; ***p < 0.001, ****p < 0.0001 (two-way ANOVA with repeated measures and the Bonferroni post hoc test; ASA or fluvastatin vs . control vehicle).

Article Snippet: In brief, after stimulation, platelets were resuspended in Annexin Binding Buffer (MabTag, Oldenburg, Germany) for immunostaining.

Techniques: Binding Assay, Concentration Assay, Expressing, Flow Cytometry, Negative Control, Positive Control, Control

Journal: Nature Chemical Biology

Article Title: Menin-MLL Inhibitors Reverse Oncogenic Activity of MLL Fusion Proteins in Leukemia

doi: 10.1038/nchembio.773

Figure Lengend Snippet: (a) MTT cell viability assay in the MLL leukemia cells KOPN-8 and MV4;11 induced by MI-2, MI-3 and MI-nc after 72h treatment. Non-MLL leukemia cell line ME-1 is shown for comparison. Data represent mean values for four samples ± s.d. Experiment was performed three times. (b) Apoptosis and cell death induced by MI-2, MI-3 and MI-nc in MV4;11 cells as detected by flow cytometry using AnnexinV/propidium iodide (PI) staining. Data represent mean values for triplicates ± s.d. (c) Selected histograms from cell cycle analysis performed by FACS after PI staining in MV4;11 cells treated with DMSO, MI-2 or MI-nc. (d) Dose-dependent effect of MI-2 on cell cycle progression measured by FACS in MV4;11 cells after PI staining, with MI-nc as a negative control. Data represent mean values for triplicates ± s.d. (e) Wright-Giemsa stained cytospins on THP-1 and MV4;11 cells after 10 days of treatment with DMSO, MI-2 or MI-nc. (f) Detection of CD11b expression in THP-1 cells assessed by flow cytometry after 6 days of treatment with DMSO, MI-2 or MI-nc. Data represent mean values for triplicates ± s.d. (g) Expression of the HOXA9 and MEIS1 genes normalized to 18S rRNA determined by qRT-PCR in THP-1 cells treated for 6 days with MI-2 and MI-3. Data represent mean values for duplicates ± s.d. Experiment was performed three times.

Article Snippet: After incubation, 1.5×10 5 cells were harvested and resuspended in 100 μl 1× Annexin V binding buffer from the Annexin V-FITC Apoptosis kit (BD Biosciences Pharmingen), incubated with 4 μl of AnnexinV-FITC and 6 μl of Propidium iodide (Sigma-Aldrich) at room temperature in the dark for 10 minutes and analyzed by flow cytometry on a LSR II instrument.

Techniques: Viability Assay, Flow Cytometry, Staining, Cell Cycle Assay, Negative Control, Expressing, Quantitative RT-PCR